rabbit anti psd95  (Alomone Labs)

Journal: Frontiers in Endocrinology

Article Title: Dorzagliatin shows potential in preventing cognitive impairment in diabetes: evidence from Mendelian randomization analysis and animal study

doi: 10.3389/fendo.2025.1755359

Figure Lengend Snippet: Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

Article Snippet: The membranes were blocked with 1×TBST and 5% BSA (tank blotting) or 5% skim milk (Semi-dry blotting) for 1 hour at room temperature and then incubated overnight at 4°C with following primary antibodies, respectively: mouse monoclonal anti-GluN1 antibody (Millipore, Cat. No: 05-432); rabbit polyclonal anti-GluN2A antibody (NOVUS, Cat. No: NB300-105); rabbit monoclonal anti-GLUT1 antibody (Abcam, Cat. No: ab115730); rabbit polyclonal anti-GLUT3 antibody (Bioss, Cat. No: bs-1207R); rabbit polyclonal anti-IR antibody (Abcam, Cat. No: ab137747); rabbit monoclonal anti-PSD95 antibody (Abcam, Cat. No: ab238135).

Techniques: Western Blot, Control, Two Tailed Test

Journal: Bioactive Materials

Article Title: Bioengineering an improved three-dimensional vascularized co-culture model for studying Neuron–Microglia interactions

doi: 10.1016/j.bioactmat.2025.09.008

Figure Lengend Snippet: Enhanced neuronal differentiation and vascular-neuronal interactions in oriented donut-shaped SF scaffolds via hiNSCs and hVOs co-culture. (A) Immunofluorescence staining showing intermingled TUJ1-positive neurons (red) and CD31-positive hVOs (green) within the hydrogel-based co-culture. Scale bar, 100 μm. (B) Semi-quantitative analysis showing increased number and length of neuronal protrusions in the co-culture group compared to the mono-culture group (n = 7 and n = 4). (C) Pattern diagram showing the construction of a hiNSCs–hVOs co-culture model on a SF scaffold. (D) Representative immunofluorescence image showing increased neurite outgrowth in hiNSCs after co-culture with hVOs. Scale bar, 200 μm. (E) Spatial overlap of CD31-positive hVOs and TUJ1-positive neurons within the scaffold, with hVOs-derived structures extending outward from the central region along radial pores. Scale bar, 100 μm. (F) RT-qPCR analysis showing upregulated expression of NFAT , GAP-43 , and CREST genes in the co-culture group compared to the mono-culture group (n = 3). (G–H) Representative Western blot images and quantification showing increased expression of BDNF and PSD95 proteins in the co-culture group (n = 3). ∗P<0.05, ∗∗P<0.01, ∗∗∗P<0.001 .

Article Snippet: The following primary antibodies were applied overnight at 4 °C: Rabbit anti-human CD11b (1:200, Wanleibio, Shengyang, China), Rabbit anti-human CD163 (1:200, Wanleibio), Alexa Fluor® 647 mouse anti-human TUJ1 (1:200, BD biosciences, Franklin Lakes, NJ, USA), Alexa Fluor® 647 mouse anti-human CD31 (1:200, BD biosciences), Rabbit anti-human CD31 (1:200, Invitrogen, Carlsbad, CA, USA), and Rabbit anti-human PDGF Receptor β (1:100, CST, Danvers, MA, USA), Rabbit anti-human αSMA (1:200, Abcam), Rabbit anti-human COL4 (1:200, Proteintech), Rabbit anti-human BDNF (1:100, Abcam, Cambridge, UK), Rabbit anti-human PSD95 (1:100, Proteintech), Mouse anti-human IBA1 (1:200, Abcam).

Techniques: Co-Culture Assay, Immunofluorescence, Staining, Derivative Assay, Quantitative RT-PCR, Expressing, Western Blot